| Navy | The Royal Navy |
| Type | Destroyer |
| Class | Prototype |
| Pennant | D 38 |
| Built by | Yarrow Shipbuilders Ltd. (Scotstoun, Scotland) |
| Ordered | 12 Jun 1924 |
| Laid down | 8 Dec 1924 |
| Launched | 15 Jan 1926 |
| Commissioned | 15 Mar 1927 |
| End service | |
| Loss position | |
| History | HMS Ambuscade made 37.66 knots on trials and was more economical than the other prototype HMS Amazon. Sold to be broken up for scrap on 23 November 1946. Commanding Officers: Lt.Cdr. Arthur Oswald Johnson, RN Lt.Cdr. Robert Augustus Fell, RN Lt.Cdr. Edward Copson Peake, RN Lt.Cdr. Donald Emberton Mansfield, RN Lt. Harry Hutchinson, RN Lt.Cdr. John Mayling, RN A/Lt.Cdr. Harold Geeves Chesterman, DSO and Bar, RNR HMS Ambuscade is not listed as active unit in the October 1945 Navy List |
Commands listed for HMS Ambuscade (D 38)
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| Commander | From | To | |
| 1 | Lt.Cdr. Arthur Oswald Johnson, RN | 21 Mar 1940 | 14 Jun 1940 |
| 2 | Lt.Cdr. Robert Augustus Fell, RN | 14 Jun 1940 | 16 May 1942 |
| 3 | Lt.Cdr. Edward Copson Peake, RN | 16 May 1942 | 9 Nov 1942 |
| 4 | Lt.Cdr. Donald Emberton Mansfield, RN | 9 Nov 1942 | 13 Jul 1943 |
| 5 | Lt. Harry Hutchinson, RN | 13 Jul 1943 | 10 Jun 1944 |
| 6 | Lt. John Mayling, RN | 10 Jun 1944 | Feb 1945 |
| 7 | A/Lt.Cdr. Harold Geeves Chesterman, DSC, RNR | Feb 1945 | Jul 1945 ? |
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Noteable events involving Ambuscade include:
7 Dec 1940
The Dutch merchant Farmsum is torpedoed and sunk in the North Atlantic in position 52º11'N, 22º56'W. HMS Ambuscade (Lt.Cdr. R.A. Fell, RN) later picks up 15 survivors.